Plasmid carriage and the natural complexity of bacterial populations contributes to plasmid persistence

Introduction: Plasmids carry and transport genes that assist their hosts to survive in many environments. Many studies have examined the conditions for plasmid persistence bacterial populations. A limitation includes a majority of mathematical models examining only included bacteria from similar colonies. However, most cells inhabit complex communities where plasmids disseminate between varied host cells. Thus, there is gap knowledge concerning natural To address few these gaps knowledge, present study attempted examine effects carriage on intrinsic stages populations Bacillus subtilis co-cultures. Material methods: B. were transformed with CRISPR-hCas-9 vectors phases growth, biofilm production, antibiotic resistance relation carriage. These three measured through vitro co-culture assays. Results: After calculating CFU/mL, growth subtilis-Carrier Escherichia coli (B. sub-C-E. coli) Vibrio harveyi sub-C-VH) co-cultures significantly decreased paired-t-test two-tailed P=0. The WT subtilis-V.H samples, Carrier-V.H co-cultures, controls each scored total 40, 47, 46 crystal violet (CV) intensity biofilm, respectively. Biofilm formation after co-culturing E. subtilis-Carrier, yielding P<0.001. levels increased by 3% sub-C-V.H samples while sensitivity approximately 1.5%. Conclusions: Plasmid contributes via altering